Journal: Journal of Neuroscience
Article Title: Neurological and Neurodegenerative Alterations in a Transgenic Mouse Model Expressing Human -Synuclein under Oligodendrocyte Promoter: Implications for Multiple System Atrophy
doi: 10.1523/jneurosci.3527-05.2005
Figure Lengend Snippet: Figure3. ComparisonoftheglialcellinclusionsbetweenMSAandMBPh-syntganimals.Imagesarefromthewhitematter tractsinthebasalgangliaofahumancasewithtypicalMSAandMBPh-syntgmicefromline1(4monthsofage).A,Conicaland ovoid GCIs in an MSA case were positively immunostained with a polyclonal antibody against h-syn (72-10). B, GCIs immuno- stained with a monoclonal antibody against h-syn (LB509). C, GCIs immunostained with a monoclonal antibody against phospho-serine129 h-syn (pser129). D, GCIs immunostained with an antibody against ubiquitin. E, Conical and ovoid glial inclusionsinanMBPh-syntgmousewerepositivelyimmunostainedwithapolyclonalantibodyagainsth-syn(72-10).F,Glial inclusionsimmunostainedwithamonoclonalantibodyagainsth-syn(LB509).G,Glialinclusionsimmunostainedwithamono- clonalantibodyagainstphospho-serine129h-syn(pser129).H,Glialinclusionsinthetgmiceweremildlyimmunostainedwith an antibody against ubiquitin. Scale bar, 20 m.
Article Snippet: Twenty micrograms of cytosolic fraction per mouse were loaded onto 10% Bis-Tris (Invitrogen) SDSPAGE gels, transferred onto Immobilon membranes, incubated with a rabbit polyclonal anti-h -syn antibody (72-10; 1:1000) (Masliah et al., 2000) followed by an anti-rabbit secondary antibody, reacted with ECL, and developed on a VersaDoc gel imaging machine (Bio-Rad, Hercules, CA).
Techniques: Staining, Ubiquitin Proteomics